Journal: The Journal of Experimental Medicine

Article Title: β 1 Integrins Are Critically Involved in Neutrophil Locomotion in Extravascular Tissue In Vivo

doi:

Figure Lengend Snippet: Frequency distribution of PMN migration velocity in extravascular tissue of the rat mesentery in response to chemotactic stimulation with PAF (10 −7 M). ( A ) Migration velocities during control period (PAF alone). ( B ) Migration velocities after topical treatment with the anti-β 1 mAb HMβ1-1.

Article Snippet: The following antibodies were used: mAb HMβ1-1 ( PharMingen , San Diego, CA) and purified IgG from a rabbit antiserum (gift of K. Rubin, Uppsala, Sweden) against the rat β 1 chain (CD29); mAbs CL26 (Upjohn, Kalamazoo, MI) and WT.3 ( PharMingen ), recognizing the rat β 2 chain (CD18); mAbs MRα4 ( PharMingen ) and TA-2 (Serotec, Oxford, UK), which react with the rat integrin α 4 subunit (CD49d); mAb HMα5-1 against the rat α 5 (CD49e) ( PharMingen ); and a rabbit antiserum against human α v β 3 (CD51/CD61) ( ).

Techniques: Migration

Journal: The Journal of Experimental Medicine

Article Title: β 1 Integrins Are Critically Involved in Neutrophil Locomotion in Extravascular Tissue In Vivo

doi:

Figure Lengend Snippet: Time course of inhibitory effect of the anti-β 1 mAb HMβ1-1 on PMN migration velocity in the rat mesentery (•) compared with control antibody (○). Data are based on calculation of mean migration velocity during defined 10 or 20 min intervals, and presented as means ± SD of five separate experiments for each antibody. Note that the inhibitory effect on PMN locomotion persisted throughout the observation period.

Article Snippet: The following antibodies were used: mAb HMβ1-1 ( PharMingen , San Diego, CA) and purified IgG from a rabbit antiserum (gift of K. Rubin, Uppsala, Sweden) against the rat β 1 chain (CD29); mAbs CL26 (Upjohn, Kalamazoo, MI) and WT.3 ( PharMingen ), recognizing the rat β 2 chain (CD18); mAbs MRα4 ( PharMingen ) and TA-2 (Serotec, Oxford, UK), which react with the rat integrin α 4 subunit (CD49d); mAb HMα5-1 against the rat α 5 (CD49e) ( PharMingen ); and a rabbit antiserum against human α v β 3 (CD51/CD61) ( ).

Techniques: Migration

Journal: International Journal of Molecular Sciences

Article Title: Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 Expression

doi: 10.3390/ijms23031240

Figure Lengend Snippet: Effects of periostin (POSTN) silencing on integrin-signaling-pathway-related protein. Western blotting assay was performed to detect integrin-αvβ3, p-AKT, total AKT, and PI3K in A549.shRNA cells compared to A549.CTRL cells. β-actin protein was used as loading controls.

Article Snippet: The following antibodies were used: primary rabbit anti-human POSTN polyclonal antibody (NBP1-82472; Novus Biologicals, Littleton, CO, USA) diluted at 1:500, rabbit anti-human MMP-2 antibody (10373-2-AP Proteintech, Manchester, UK) diluted at 1:1000, rabbit anti-human α V β 3 antibody (NBP2-67557; Novus Biologicals) diluted at 1:1000, rabbit anti-human pAKT antibody (#4058 Cell Signaling Technology, Danvers, MA, USA), diluted at 1:1000, rabbit anti-human AKT antibody (#9272 Cell Signaling Technology) diluted at 1:1000, and mouse anti-human PI3K antibody (MA5-17149, Thermo Fisher Scientific, Carlsbad, CA, USA) diluted at 1:1000.

Techniques: Western Blot, shRNA